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Thu Mar 08, 2012 8:36 pm  |
lol....actually...yes it is Yak meat tenderizer as well. Multi purpose.
Recent advances in characterizing biological mechanisms underlying UV-induced wrinkles: a pivotal role of fibrobrast-derived elastase.
Imokawa G.
Source
Katayanagi Institute-W204, School of Bionics, Tokyo University of Technology, 1404-1 Katakura Hachioji, Tokyo 192-0982, Japan. imokawa@bs.teu.ac.jp
Abstract
In clinical studies, the formation of facial wrinkles has been closely linked to the loss of elastic properties of the skin. Cumulative irradiation with ultraviolet (UV) B at suberythemal doses significantly reduces the elastic properties of the skin, resulting in the formation of wrinkles. In in vitro studies, we identified a paracrine pathway between keratinocytes and fibroblasts, which leads to wrinkle formation via the up-regulation of fibroblast elastases that degrade elastic fibers. UVB irradiation stimulates the activity of fibroblast elastases in animal skin. Scanning electron microscopy revealed that cumulative UVB irradiation elicits a marked alteration in the three-dimensional structure of elastic fibers, which is closely associated with the subsequent reduction in the elastic properties of the skin, resulting in wrinkle formation. Studies using anti-wrinkle treatments suggest a close relationship between the recovery of wrinkles and an improvement in the linearity of elastic fibers. Those studies also suggest a close correlation between the recovery in the linearity of elastic fibers and the improvement in skin elasticity. In a study using ovariectomized animals, we characterized the important role of elastase in their high vulnerability to UV-induced wrinkle formation. A synthetic inhibitor specific for fibroblast elastases significantly prevents wrinkle formation without reducing the elastic properties of the skin, accompanied by minor damage in elastic fibers. Finally, we identified an effective extract of Zingiber officinale (L.) Rose from a screen of many herb extracts, which has a safe and potent inhibitory activity against fibroblast elastases. Animal studies using the L. Rose extract revealed that it has significant preventive effects against UVB-induced wrinkle formation, which occur in concert with beneficial effects on skin elasticity. A 1-year clinical study on human facial skin to determine the efficacy of the L. Rose extract demonstrated that it inhibits the UV-induced decrease in skin elasticity and prevents or improves wrinkle formation in skin around the corner of the eye without changing the water content of the stratum corneum. Our long-term studies support our hypothesis for a mechanism of wrinkle formation in which cytokine expression is activated by UV irradiation and triggers dermal fibroblasts to increase the expression of elastase. That increase in elastase results in the deterioration of the three-dimensional architecture of elastic fibers, reducing skin elasticity and finally leading to the formation of wrinkles.
Smells not bad either^^^. Works well with Neroli, Ylang ylang and Frankincense.
Lotusesther: Soy Isoflavones -Genistein
http://www.ajcn.org/content/87/5/1314.full.pdf
http://www.ornatural.com/eng/pdf/detox-slim/Genistein%20-%20SOFW%20Journal.pdf |
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Sat Mar 17, 2012 10:40 am |
| Lotusesther wrote: |
| very high concentrations. |
Not quite...the extract needs to be highly concentrated.
| Lotusesther wrote: |
| What does this mean for DIY? How difficult is it to get it into a usable form, at a sufficient concentration? I have a kitchen, not a lab. Another blond question: if it takes 2 steps to turn soy isoflavones into something that works, why not sell processed isoflavones that are already usable? Or only need step 2? |
You ask good questions you know that?
1. Means, it is doable.
2. Define difficult? It's not. Purified extract, = you don't need much. Less than 1% raw material.
3. Perfect. Just need some basic equipment.
4. Cancer drug pharmacology. Taking advantage of the simple two step process omitting re-crystallization. |
_________________
If you make, first do no harm, your Law, you will never strike the first blow and will be known as a man of peace who can fight like ten tigers, a Human in the act of Being. There is no greater rank than this. Ashida Kim on War.~Cellese~AnteAge Serum and Accelerator, DermaRoller ,MyFawnie AA2G serum, KNN G ForceUltrasound., SEA, ChrySun 25% ZnO |
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Sun Apr 01, 2012 5:31 am |
Lotusesther,
My mother went into early meno, finished at 38. Hyper/hypothyroidism in the mix. Has done a terrible number on her skin.
B3 5%
NAG 2%
Genistein 1%Genistein protects against UVB-induced senescence-like characteristics in human
dermal fibroblast by p66Shc down-regulation
Proline 1%
Carnosine 1%
AA2G 2%
AHYP 2%
Vit E 1%
Retinol 1 %
CoQ 10 2%
Lecithin 5%
Ewax-NF 7 %
Poly 80 2%
Oils 13 %
Coca butter 7 %
Shea 7 %
Glycerin 3 %
1 N NaOH 2%
H2O 36 %
Phenoxy 0.5 %
pH 5.5
That is an exceptionally hydrating formula without being sticky or greasy. Peach colored luxury, that when rubbed turns white and is rapidly absorbed into the skin. Leaves my skin butter soft.
Light reading:
FACT SHEET on the PHYTOESTROGEN GENISTEIN
Potentially quite useful due to the estrogen mimic effect , although weak, has been shown in vitro and in vivo to be promising.
It is Not the Fountain of Youth in a Cheap Plastic Bottle [nothing is]...but is quite helpful.
And...tossing it into water/lotion/ cream will be of absolutely Zero use to you. Genistein is the only " tricky" one. CoQ10 goes into your oil. Oil soluble. The rest are water soluble. Easy to make. |
_________________
If you make, first do no harm, your Law, you will never strike the first blow and will be known as a man of peace who can fight like ten tigers, a Human in the act of Being. There is no greater rank than this. Ashida Kim on War.~Cellese~AnteAge Serum and Accelerator, DermaRoller ,MyFawnie AA2G serum, KNN G ForceUltrasound., SEA, ChrySun 25% ZnO |
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Sun Apr 01, 2012 7:30 am |
Thank you DM.RE: AA2G, It's quite versatile. However the pH in solution is around 2.1.
| Lotusesther wrote: |
| That would suggest adding it to the water phase of any DIY lotion should do the trick? |
Would be nice were that the case. Unfortunately the amount present is quite low. And essentially unknown as it is a composite that is given as the %.
90mg/kg in vivo for effect using Genistein. Penetration is about half that even when combined with penetration enhancers. However over 100mg/Kg ...you have cell death.
It's a balancing act.
AHYP 2% N-acetyl L hydroxyproline has greater penetration and is generally less irritating than L Hydroxyproline when applied topically. Funny and cruel aspect for women is that our males at the age of 50 retain higher estrogen levels than we do which apparently plays an important role in skin condition. L-hydroxproline levels are significantly reduced in older women compared to their youth, as a result of the lower estrogen level and the enzyme mediated activities of the hormone.
Based on some interesting research from a while back, steric hindrance may play a role as well in reduction of collagenase activity, however that remains unconfirmed. AHYP regardless of that, still remains beneficial from the hydration aspect.i.e. rather than applying hyaluronic acid to the skin to increase hydration levels, the skin can be induced to create own. Combined with B3, more so.
| Lotusesther wrote: |
| You use NAOH, I must say I feel somewhat reluctant about that. |
"The big scare"...nothing to fear with that. Just don't drink it. Pour it in your eyes or anything stupid of that nature.
1 N NaOH = 0.39 grams NaOH dissolved in 10 grams distilled water.
1 M Citric Acid = 1.92 grams C3H4OH(COOH)3 dissolved in 10 grams distilled water
Citric acid + Sodium Hydroxide----> Sodium citrate salt + water
1N NaOH then a little EtOH on the mix...and increase the bio availability 10X.
I am guilty of loading my bases Lotusesther. I don't use Soy isoflavones, I use Genistein, because either way, you will require the same solvent to hope to gain anything out of it. The bonus, Genistein doesn't stink like the soy isoflavones tend to. |
_________________
If you make, first do no harm, your Law, you will never strike the first blow and will be known as a man of peace who can fight like ten tigers, a Human in the act of Being. There is no greater rank than this. Ashida Kim on War.~Cellese~AnteAge Serum and Accelerator, DermaRoller ,MyFawnie AA2G serum, KNN G ForceUltrasound., SEA, ChrySun 25% ZnO |
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Sun Apr 01, 2012 11:56 am |
| Thank you Dark Moon!!! |
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Mon Apr 02, 2012 7:25 am |
It is interesting to see Vitamin C paired with a known glycating agent (glucose)...I am sure there is an explanation for that, one that's over my head for now.
I currently use Ovestin topically on the face.
I also use my own cocktail serum for post dermarolling - containing red palm oil and other vitamins and antioxidants (all oil soluble). It would appear to be of little benefit to attempt adding some form of soy to this serum?
Do I understand that correctly?
Thanks, BFG |
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Mon Apr 02, 2012 7:30 am |
Was $75.00 US for 5 grams from a US supplier so if you are getting it at $30.00 for 15 grams...post the link. I'd love to get it for that price. 
| DarkMoon wrote: |
I assure you Dragon I read what I posted and I am well aware of the pH of water, but I will say at times your posts for those of us not chemists are as clear as mud.
I was questioning this:
| DragoN wrote: |
| Thank you DM.RE: AA2G, It's quite versatile. However the pH in solution is around 2.1. |
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I am certain that you do. I don't question that. In solution = the AA2G only, in water. The pH is very low. More options for formulating given the stability and the pH range. I like it. |
_________________
If you make, first do no harm, your Law, you will never strike the first blow and will be known as a man of peace who can fight like ten tigers, a Human in the act of Being. There is no greater rank than this. Ashida Kim on War.~Cellese~AnteAge Serum and Accelerator, DermaRoller ,MyFawnie AA2G serum, KNN G ForceUltrasound., SEA, ChrySun 25% ZnO |
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Mon Apr 02, 2012 7:48 am |
Ovestin sounds a lot less trouble
Thnx DragoN and DarkMoon! But at $75 per 5 grams, and p & p on top of that.. and my experience with L-AA was not a nice one (and that where I am never ever oversensitive to anything!)so I don't think I am going to try that for now.
And THANKS A LOT!!! for all the very interesting info on the other ingredients, some of which I had never heard of! |
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Mon Apr 02, 2012 12:08 pm |
| DarkMoon wrote: |
| It would be a personal favor by this person to me, I will not take advantage of a private situation by divulging anything |
oh goodness....lucky you!!
Fortunately, when I Googled "AA2G DIY skincare"
I found it!!!! Thank you sooo much!
Genistein too
Genistein protects against UVB-induced senescence-like characteristics in human dermal fibroblast by p66Shc own-regulation
This is not an ingestion study.... 
Effects of the isoflavone 4′,5,7-trihydroxyisoflavone (genistein) on psoralen plus ultraviolet A radiation (PUVA)-induced photodamage
..neither is that one.
The Equol price is a little steep though:
10 g = $1190.00
Maybe your friend can get you a better deal on that? |
_________________
If you make, first do no harm, your Law, you will never strike the first blow and will be known as a man of peace who can fight like ten tigers, a Human in the act of Being. There is no greater rank than this. Ashida Kim on War.~Cellese~AnteAge Serum and Accelerator, DermaRoller ,MyFawnie AA2G serum, KNN G ForceUltrasound., SEA, ChrySun 25% ZnO |
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Wed Apr 04, 2012 3:09 pm |
| daler wrote: |
| gretchen wrote: |
| I wouldn't touch soy with a ten foot pole. |
why would you say that... Asians eats lots of soy based products and as far as I know most of em live a very healthy n long life.... |
My husband is half-Japanese and the soy consumed by many Asians isn't the soy that most of us are used to..they eat fermented soy like Natto & Miso. The soy that most of us in America is used to seeing are actually highly processed unfermented soy products. |
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Sat Apr 07, 2012 3:00 pm |
| Barefootgirl wrote: |
Thanks DM, I do not own that particular textbook, although I would like to.
BFG |
Here ya go BFG, in pdf form:
http://uploaded.to/file/kzqcjz8o
Click on Free Download. Lots of medical texts are available as free downloads so that we dont have to take anyone's word for it - pays to be an informed consumer. |
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Treespider
New Member
 
Joined: 12 Apr 2012
Posts: 2
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Thu Apr 12, 2012 7:32 am |
Being an old bat, I found this pilot study quite interesting :
Effects of Isoflavones on the Skin of Postmenopausal Women: A Pilot Study
(The study can be found in PubMed..I am not allowed to post links)
This, and other articles, seem to indicate that there is a synergic effect of different soy isoflavones used topically re. UVB-induced skin damage. So, rather than using just genistein, perhaps a more complete isoflavone product has merit ? In the above study, however, I certainly miss a more exact description of the product used than just "an isoflavones-rich, concentrated soy extract".
I have a product from BulkActives with this composition :
DAIDZIN 23.80%
GLYCITIN 12.13%
GENISTIN 4.78%
DAIDZEIN 0.52%
GLYCITEIN 0.31%
GENISTEIN 0.20%
...for a total of 41.74% soy isoflavones.
What I am less sure of, is how to use it. I seem to recall Dr. J mentioning somewhere that liposomal delivery is an option...anyone remember ?
A major metabollite of daidzein is equol. The good results from Asia may have something to do with the Asian population's higher percentage of equol-producing individuals as compared to western populations. If I knew I was an equol-producer, maybe I would eat this stuff instead... |
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Thu Apr 12, 2012 7:41 am |
| Treespider wrote: |
Being an old bat, I found this pilot study quite interesting :
Effects of Isoflavones on the Skin of Postmenopausal Women: A Pilot Study
(The study can be found in PubMed..I am not allowed to post links)
This, and other articles, seem to indicate that there is a synergic effect of different soy isoflavones used topically re. UVB-induced skin damage. So, rather than using just genistein, perhaps a more complete isoflavone product has merit ? In the above study, however, I certainly miss a more exact description of the product used than just "an isoflavones-rich, concentrated soy extract".
I have a product from BulkActives with this composition :
DAIDZIN 23.80%
GLYCITIN 12.13%
GENISTIN 4.78%
DAIDZEIN 0.52%
GLYCITEIN 0.31%
GENISTEIN 0.20%
...for a total of 41.74% soy isoflavones.
What I am less sure of, is how to use it. I seem to recall Dr. J mentioning somewhere that liposomal delivery is an option...anyone remember ?
A major metabollite of daidzein is equol. The good results from Asia may have something to do with the Asian population's higher percentage of equol-producing individuals as compared to western populations. If I knew I was an equol-producer, maybe I would eat this stuff instead... |
Here is the link to the study:
http://www.ncbi.nlm.nih.gov/pubmed?term=%20Effects%20of%20Isoflavones%20on%20the%20Skin%20of%20Postmenopausal%20Women%3A%20A%20Pilot%20Study%20 |
_________________
I'LL SEE YOU ON THE DARKSIDE OF THE MOON.... |
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Thu Apr 12, 2012 7:42 pm |
Effects of Isoflavones on the Skin of Postmenopausal Women: A Pilot Study
Alfeu Accorsi-Neto,I Mauro Haidar,I Ricardo Simões,I Manuel Simões,II José Soares-Jr,IIII and Edmund BaracatIIIIV
IGynecology Department, Federal University of São Paulo (UNIFESP) - São Paulo/SP, Brazil
IIMorphology Department, Federal University of São Paulo (UNIFESP) - São Paulo/SP, Brazil
IIILIM-58, Laboratório de Ginecologia Estrutural e Molecular, Faculdade de Medicina da Universidade de São Paulo - São Paulo/SP, Brazil
IVDisciplina de Ginecologia, Departamento de Obstetrícia e Ginecologia, Faculdade de Medicina da Universidade de São Paulo - São Paulo/SP,Brazil. Study performed at: Gynecology Department of the Federal University of São Paulo (UNIFESP) - São Paulo/SP, Brazil
Email: jsoares415@hotmail.com, Tel: 55 11 5081-3685
Received January 25, 2009; Accepted March 2, 2009.
Small right arrow pointing to: This article has been cited by other articles in PMC.
Other Sections▼
Abstract
OBJECTIVE:
The aim of this study was to evaluate the effects of isoflavones on the skin of postmenopausal women.
DESIGN:
A prospective study was performed with 30 postmenopausal women before and immediately after the end of treatment with 100 mg/day of an isoflavones-rich, concentrated soy extract for six months. A skin punch was performed in the gluteal region for sample collection before and immediately after the treatment program. Morphometric determination of epidermal thickness, the papillary index (wrinkling), and the amount of dermal elastic and collagen fibers was assessed. In addition, the number of blood vessels in the sample was also evaluated. The paired Student’s t-test was used for statistical analysis (P ≤ 0.05).
RESULTS:
Isoflavone treatment resulted in a 9.46% increase in the thickness of the epidermis in 23 patients. In addition, the papillary index was reduced in 21 women. The papillary index was inversely proportional to skin wrinkling, i.e., there were a large number of papillae after treatment. The amount of collagen in the dermis was increased in 25 women (86.2%). In 22 women (75.8%) we observed that the number of elastic fibers increased. The number of dermal blood vessels was significantly increased in 21 women.
CONCLUSION:
Our data show that the use of a concentrated, isoflavone-rich soy extract during six consecutive months caused significant increases in epithelial thickness, the number of elastic and collagen fibers, as well as the blood vessels.
Ingestion study of concentrated soy extract 100mg/day.
DAIDZIN 23.80%
GLYCITIN 12.13%
GENISTIN 4.78%
DAIDZEIN 0.52%
GLYCITEIN 0.31%
GENISTEIN 0.20%
...for a total of 41.74% soy isoflavones. And the rest is a mix of soy protein.
You need close to 1% of the active component for topical effect. Run the numbers.
Topical application of Bifidobacterium-fermented soy milk extract containing genistein and daidzein improves rheological and physiological properties of skin
And in the above it was fermented soy milk 10% in EtOH.
| Treespider wrote: |
| If I knew I was an equol-producer, maybe I would eat this stuff instead... Wink |
Would get more out of it...but you will still get zero out of it topically applied at those concentrations even with genistein present. |
_________________
If you make, first do no harm, your Law, you will never strike the first blow and will be known as a man of peace who can fight like ten tigers, a Human in the act of Being. There is no greater rank than this. Ashida Kim on War.~Cellese~AnteAge Serum and Accelerator, DermaRoller ,MyFawnie AA2G serum, KNN G ForceUltrasound., SEA, ChrySun 25% ZnO |
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Sat May 26, 2012 9:41 am |
Lipobelle Soyaglycone
Isoflavones in their most active form
Lipobelle Soyaglycone is a liposomal preparation of genistein, the bioactivated form of a soy isoflavone. In skin, isoflavones have been shown to stimulate the production of the structural components, collagens, and to inhibit matrix degrading enzymes.
Lipobelle Soyaglycone contains the isoflavones in their physiologically most active form, called aglycone. For penetration into deeper viable skin layers, the isoflavone aglycones (genistein) have been incorporated into a liposomal preparation. In the dermis, Lipobelle Soyaglycone boosts collagen production, which leads to increased skin density and improved skin firmness and elasticity.
Statistically significant studies show that Lipobelle Soyaglycone is a true collagen booster when it is formulated in a liposomal preparation.
Claims with Lipobelle Soyaglycone
Improves skin density, firmness and elasticity
Plumps up fine lines from inside
Skin looks more lifted
Anti-gravity effect
.....Genistein...nice active
Not so sure about the "anti gravity" aspect . 
It has other benefits though. |
_________________
If you make, first do no harm, your Law, you will never strike the first blow and will be known as a man of peace who can fight like ten tigers, a Human in the act of Being. There is no greater rank than this. Ashida Kim on War.~Cellese~AnteAge Serum and Accelerator, DermaRoller ,MyFawnie AA2G serum, KNN G ForceUltrasound., SEA, ChrySun 25% ZnO |
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Fri Jun 15, 2012 2:14 pm |
The estrogenic activity of soy isoflavones is not very high, and the efficacity of creams like the soy cream for things like hot flushes is barely more than a placebo. The other ingredients dong quai and black cohosh are according to my herbals used as 'hormone balancer', black cohosh is something you would really want to research carefully before taking it as a supplement.
http://circ.ahajournals.org/content/113/7/1034.short |
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Fri Jun 15, 2012 2:24 pm |
| fawnie wrote: |
| egyptiangoddess wrote: |
Does anyone know if I could add soy isoflavones to a DIY spritz? I'm wanting it for soothing/lightening/collagen stimulating properties and soy is good for my rosacea. I'm specifically talking about the 40% soy isoflavones from bulkactives. It says it's water soluble. But then people say it stinks.  I've seen the 98% genistein on sea, but I'm not sure that would have the same lightening/soothing properties. (From what I've read, it seems genistein is more just for collagen stimulating?) |
Sorry EG I missed that I guess. 40% soy isoflavones have how much genistein? Genistein is the main active in isoflavones so that is the one we are concerned about no matter what you plan to use isoflavones for. "Genistein exhibits antioxidant, antiangiogenic, and immunosuppressive activities."
You could try either one I guess but the 98% genistein has to be dissolved in NaOH and then ETOH so its a bit tricky. So if you want an easy one, the water sol would be your choice. |
Also, in case you do decide to use NaOH, please be very careful and use protective goggles n all.. it' s a very dangerous n should be used by experienced people... |
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Fri Jun 15, 2012 2:37 pm |
Thank you everyone, so much! I hope adding soy to my spritz works.
Thank you DM, I just wasn't sure if it would really dissolve enough to work in a spray bottle!
Lotus, I'm not taking it as a supplement. Just wanting to use it topically in a spritz. Topical soy is great for rosacea, sensitive skin and discoloration. My skin loves soy. 
Thanks fawnie!! I sure hope it works and doesn't stink lol!
Thanks for the tip daler, I'm brand new to DIY so I'm definitely not ready to work with NaOH yet.
Thanks again everyone. |
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Velicia
New Member
Joined: 16 Mar 2012
Posts: 1
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Thu Dec 06, 2012 8:26 pm |
| Lotusesther wrote: |
| Fawnie, how do you make liposomes in, say, a kitchen? |
Hi, All... I am a first-time poster, but have been lurking since March. I am dazzled by the wealth of information here, so kudos to you all.
Lotusesther, insofar as liposomes, I have recently become familiar with a DIY technique that can be done "in a kitchen", and rather inexpensively, at that. Please be advised that I have not yet tried this, but am ramping up to do so.
The following is a cut-and-paste of the technique as explained by Brooks Bradley, the technique's developer (I'd include the link to the source, but apparently new members are not allowed to do so). It is intended for oral consumption, but the moderator of the DIY-LET ("Liposomal Encapsulation Technology") Yahoo group I recently joined asserts that the process should yield a product of superior efficacy for topical application. My knowledge is too unsophisticated at this point for me to be critical of his claim, but I can't see how it would hurt to try it out. I'm thinking I'll use the technique in the 'C' serum recipe that also uses camu camu powder, which I've also seen in these forums. I believe some were saying how difficult to is to dissolve the camu powder, so perhaps the ultrasonic cleaner can alleviate this problem. I intend to see if the technique will also enhance the delivery of CoQ10, turmeric, etc. I'm not sure if its wise to do all of this in one serum... I'd hate to be responsible for creating a mushroom cloud on the horizon <wink>.
Brooks Bradley:
| Quote: |
Our vitamin "C" liposomal encapsulation protocol is as follows:
Using a small (2 cup) Ultrasonic cleaner, (Item #03305, obtainable from Harbor Freight @ about $30.00), we performed the following:
1. Dissolved 3 level tablespoons of soy lecithin in 1 cup of water (preferably distilled).
2. Dissolved 1 level tablespoon of ascorbic acid powder (Vit. "C") in 1/2 cup
of water.
3. Poured both solutions together in the ultrasonic cleaner bowl and turned the unit on. Using a plastic straw (leaving the top of the cleaner opened), gently, slowly, stirred the contents.
Note: The cleaner will, automatically, self-stop about every 2 minutes. Just push ON button to continue. Repeat for a total of 3 series (6 minutes). By that time the entire solution should be blended into a cloudy, homogeneous, milk-like mixture. The LET solution is now formed.
4. This protocol furnishes about 12 grams (12000mg.) of vitamin C product. At 70% encapsulation efficiency, 8400 mg would be of the LET type. This solution will keep, acceptably, at room temperature for 3 to 4 days. Refrigerated, it will keep much longer. We use it so fast around our place...there isn't enough left to be concerned over storage. The "homogenizing effect" is so powerful that after 3 days at room temperature, no precipitation or solution separation appears evident. This type of sequestered vitamin "C" has demonstrated to be, at least 5 times more effective (per volumetric measure) than any other form of orally-ingested vitamin "c"....that we have tested. Additionally, it appears to be even more rapid in tissue-bed availability----than IV applications. An astounding revelation....to us. We estimate the DIY researcher can produce the active LET portion of this solution for 15 cents per gram....as against about $1.00 per gram from commerci! al sources.
It is my hope that this, limited, explanation of our activities in this area,
is of some value to our do-it-yourself health-maintenance researchers. In any event, this protocol has demonstrated to be n on-toxic and most helpful to OUR RESEARCHES. |
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